Anti-RSPO1 antibody (ab106556)
Key features and details
- Rabbit polyclonal to RSPO1
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RSPO1 antibody
See all RSPO1 primary antibodies -
Description
Rabbit polyclonal to RSPO1 -
Host species
Rabbit -
Specificity
At least three isoforms of RSPO1 are known to exist; ab106556 will detect the largest and smallest isoforms. ab106556 will not cross-react with other RSPO family members. -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human RSPO1 (N terminal).
Database link: Q2MKA7 -
Positive control
- Rat spleen tissue lysate; Human spleen tissue, Human heart tissue lysate
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General notes
Antibodies should not be exposed to prolonged high temperatures.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry of 4% paraformaldehyde-fixed human spleen labeling RSPO1 with 5171 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).
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Lane 1 : Anti-RSPO1 antibody (ab106556) at 0.5 µg
Lane 2 : Anti-RSPO1 antibody (ab106556) at 1 µg
All lanes : Human spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa
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All lanes : Anti-RSPO1 antibody (ab106556) at 2 µg
Lane 1 : Daudi cell lysate
Lane 2 : 3T3/NIH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa
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Lane 1 : Anti-RSPO1 antibody (ab106556) at 1 µg/ml
Lane 2 : Anti-RSPO1 antibody (ab106556) at 2 µg/ml
All lanes : Human heart tissue lysate
Predicted band size: 29 kDa
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Immunohistochemical analysis of PFA fixed paraffin embedded Human spleen tissue sections labelling RSPO1 with ab106556, at 2.5 µg/mL.
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Lane 1 : Anti-RSPO1 antibody (ab106556) at 1 µg/ml
Lane 2 : Anti-RSPO1 antibody (ab106556) at 2 µg/ml
All lanes : Rat spleen tissue
lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 29 kDa
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Immunohistochemical analysis of PFA fixed paraffin embedded Human heart tissue sections labelling RSPO1 with ab106556, at 2.5 µg/mL.