Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6959] to N WASP - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-N WASP antibody [EPR6959] - BSA and Azide free
    See all N WASP primary antibodies

  • Description

    Rabbit monoclonal [EPR6959] to N WASP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, wild-type and N WASP knockout HAP1 cell lysate; Human thyroid tissue lysate.

  • General notes

    Ab232457 is the carrier-free version of ab126626. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232457 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)
    Western blot - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: N WASP knockout HAP1 cell lysate (20 µg)
    Lane 3: A549 cell lysate (20 µg)
    Lane 4: Human thyroid tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab126626 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab126626 was shown to recognize N WASP when N WASP knockout samples were used, along with additional cross-reactive bands. Wild-type and N WASP knockout samples were subjected to SDS-PAGE. ab126626 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126626).

  • Flow Cytometry - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)
    Flow Cytometry - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)

    Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling N WASP with purified ab126626 at 1/150 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126626).

  • Immunocytochemistry/ Immunofluorescence - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)
    Immunocytochemistry/ Immunofluorescence - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)

    Immunofluorescence staining of K562 cells with purified ab126626 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab126626 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126626).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)

    Unpurified ab126626, at 1/50, staining N WASP in formalin fixed, paraffin embedded human papillary carcinoma tissue by Immunohistochemistry

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126626).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)
    OI-RD Scanning - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126626).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)

    Immunohistochemical staining of paraffin embedded human kidney with purified ab126626 at a working dilution of 1/100. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126626).

     

  • Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)
    Anti-N WASP antibody [EPR6959] - BSA and Azide free (ab232457)

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