Anti-Alpha Skeletal Muscle Actin antibody (ab52218)
Key features and details
- Rabbit polyclonal to Alpha Skeletal Muscle Actin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Alpha Skeletal Muscle Actin antibody
See all Alpha Skeletal Muscle Actin primary antibodies -
Description
Rabbit polyclonal to Alpha Skeletal Muscle Actin -
Host species
Rabbit -
Specificity
Actin alpha 1 antibody detects endogenous levels of total Actin alpha 1 protein -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic derived from human Actin alpha 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody (ab52218)
Ab52218 staining human skeletal muscle tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-Alpha Skeletal Muscle Actin antibody (ab52218)All lanes : Anti-Alpha Skeletal Muscle Actin antibody (ab52218) at 1/500 dilution
Lane 1 : extracts from LOVO cells,
Lane 2 : extracts from LOVO cells, with immunizing peptide
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody (ab52218)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Alpha Skeletal Muscle Actin with ab52218. Right pane - treated with immunizing peptide.
