Anti-Histone H4 (di methyl R3) antibody (ab194696)
Key features and details
- Rabbit polyclonal to Histone H4 (di methyl R3)
- Suitable for: WB, IHC-P, Dot blot, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Histone H4 (di methyl R3) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (di methyl R3) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Dot blot, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: a wide range of other species -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (N terminal) (di methyl R3).
Database link: P62805 -
Positive control
- WB: HeLa cell lysate; IHC-P: Human stomach, rat liver and mouse liver tissues. ICC/ IF: 293T cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Paraffin-embedded human stomach tissue stained for Histone H4 (di methyl R3) using ab194696 at 1/100 dilution in immunohistochemical analysis.
-
Immunofluorescence staining of 293T cells stained for Histone H4 (di methyl R3) with ab194696. Nuclei are labeled with DAPI (Blue).
-
All lanes : Anti-Histone H4 (di methyl R3) antibody (ab194696)
Lane 1 : HeLa cell lysate
Lane 2 : H4 protein
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 11 kDaBlocking buffer: 3% nonfat dry milk in TBST.
-
Dot-blot analysis of methylation peptides using ab194696.
-
Paraffin-embedded mouse liver tissue stained for Histone H4 (di methyl R3) using ab194696 at 1/100 dilution in immunohistochemical analysis.
-
Paraffin-embedded rat liver tissue stained for Histone H4 (di methyl R3) using ab194696 at 1/100 dilution in immunohistochemical analysis.