Anti-Histone H3 (di methyl K9) antibody (ab195482)
Key features and details
- Rabbit polyclonal to Histone H3 (di methyl K9)
- Suitable for: WB, ICC/IF, Dot blot, ChIP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (di methyl K9) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (di methyl K9) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Dot blot, ChIPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Xenopus laevis, Arabidopsis thaliana, Rice, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (di methyl K9) conjugated to keyhole limpet haemocyanin. (peptide surrounding K9)
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Positive control
- NIH 3T3 cells; Histone extracts from HeLa cells; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H3 (di methyl K9) antibody (ab195482)
ChIP analysis using HeLa cells, ab195482 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers specific for promoter of the inactive HBB gene and the coding regions of the inactive MYOD gene, used as positive controls, and for the promoters of the active genes c-fos and GAPDH, used as negative controls.
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Western blot - Anti-Histone H3 (di methyl K9) antibody (ab195482)Anti-Histone H3 (di methyl K9) antibody (ab195482) at 1/1000 dilution + Histone extracts from HeLa cells at 15 µg
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K9) antibody (ab195482)Immunofluorescent analysis of NIH 3T3 cells ( 4% formaldehyde-fixed) labeling Histone H3 (di methyl K9) with ab195482 at 1/500 dilution followed by an anti-rabbit antibody conjugated to Alexa488 (left panel). The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
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Dot Blot - Anti-Histone H3 (di methyl K9) antibody (ab195482)Dot Blot analysis was performed to test the cross reactivity of ab195482 against Histone H3 (di methyl K9) (H3K9me2) with peptides containing other modifications of histone H3 and the unmodified H3K9 sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. ab195482 was used at 1/20,000 dilution.
