Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free
See all Glutamate Receptor 1 (AMPA subtype) primary antibodies
Description

Rabbit monoclonal [EPR19522] to Glutamate Receptor 1 (AMPA subtype) - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Rat
IP	Mouse
WB	Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab250709 is the carrier-free version of ab183797. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250709 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19522
Isotype

IgG
Research areas

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)

All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/5000 dilution

Lane 1 : Mouse Glutamate Receptor 1 fragment recombinant protein
Lane 2 : Mouse Glutamate Receptor 2 fragment recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 102 kDa

This data was developed using ab183797, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 2 seconds; Lane 2: 3 minutes.

Mouse Glutamate Receptor 1 fragment recombinant protein contains aa19-184 with a GST/His-Tag^®. Mouse Glutamate Receptor 2 fragment recombinant protein contains aa25-288 with a His-Tag^®.
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)

All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat liver lysate
Lane 3 : Mouse hippocampus lysate
Lane 4 : Mouse cerebellum lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat cerebellum lysate
Lane 7 : Rat hippocampus lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 102 kDa
Observed band size: 102 kDa

This data was developed using ab183797, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-2: 30 seconds; Lanes 3-7: 15 seconds.

Rodent Glutamate Receptor 1 is widely expressed in brain and represents the predominant excitatory neurotransmitter system but not in liver.

Negative control: Rat liver (PMID: 2480522).
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)

All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] (ab183797) at 1/1000 dilution

Lane 1 : Human cerebellum lysate
Lane 2 : Human muscle lysate
Lane 3 : Human fetal brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 102 kDa
Observed band size: 102 kDa

Exposure time: 3 minutes

This data was developed using ab183797, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Human Glutamate Receptor 1 is widely expressed in brain and represents the predominant excitatory neurotransmitter system but not in muscle.

Negative control: human muscle (PMID: 1652753).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)

This data was developed using ab183797, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse hippocampus was observed [PMID: 15723058]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)

This data was developed using ab183797, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on mouse liver is observed. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)

This data was developed using ab183797, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat hippocampus is observed [PMID: 15723058]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)

This data was developed using ab183797, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on rat stomach is observed. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)

This data was developed using ab183797, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on mouse hippocampus was observed. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)

This data was developed using ab183797, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Glutamate Receptor 1 (AMPA subtype) with ab183797 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on mouse hippocampus was observed. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)

This data was developed using ab183797, the same antibody clone in a different buffer formulation.Glutamate Receptor 1 (AMPA subtype) was immunoprecipitated from 0.35 mg of Mouse brain whole cell lysate with ab183797 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183797 at 1/1000 dilution. VeriBlot for IP Detection Reaction (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse brain whole cell lysate, 10ug (Input). Lane 2: ab183797 IP in Mouse brain whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab183797 in Mouse brain whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.
Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR19522] - BSA and Azide free (ab250709)