Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23338-106] to L1CAM - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free
    See all L1CAM primary antibodies

  • Description

    Rabbit monoclonal [EPR23338-106] to L1CAM - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    ICC
    Mouse
    IHC-Fr
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain tissue lysate; Rat brain tissue lysate; PC-12 whole cell lysate. IHC-Fr: Mouse colon, cerebellum and kidney, tissue; rat colon, cerebellum and kidney tissue. ICC: PC-12, and mouse primary neuron cells. Flow cyt: PC-12, mouse primary neuron and B16-F10 cells.

  • General notes

    ab273518 is the carrier-free version of ab272733. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Western blot - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    All lanes : Anti-L1CAM antibody [EPR23338-106] (ab272733) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 140 kDa
    Observed band size: 150,250 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: NIH/3T3 (PMID: 22973895)

    L1CAM is a glycoprotein. Full length 250-kDa L1CAM and cleaved 150-kDa are observed. The molecular weight observed is consistent with what have been described in literature (PMID: 20840789, PMID: 23205105).

    Exposure time: 114 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733)

  • Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling L1CAM with ab272733 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Immunocytochemistry - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Immunocytochemistry - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Immunofluorescent analysis of 100% methanol-fixed mouse primary neuron cell cells labelling L1CAM with ab272733 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. L1CAM is specifically localized to axons, but was absent from MAP2-positive dendrites (PMID: 27001749). Negative control: NIH/3T3 (PMID: 22973895).  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain dentrites at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Flow Cytometry - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Flow Cytometry - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast)(Left) / B16-F10 (Mouse melanoma mixture of spindle-shaped and epithelial-like cells)(Right) cells labelling L1CAM with ab272733 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: NIH/3T3 (PMID: 22973895).

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling L1CAM with ab272733 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling L1CAM with ab272733 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat kidney is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Flow Cytometry - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Flow Cytometry - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Flow cytometric analysis of Mouse primary neuron cells cells labelling L1CAM with ab272733 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling L1CAM with ab272733 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse kidney is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Flow Cytometry - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Flow Cytometry - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Flow cytometric analysis of PC-12 (Rat adrenal gland pheochromocytoma) cells labelling L1CAM with ab272733 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat colon tissue labeling L1CAM with ab272733 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat colon is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon tissue labeling L1CAM with ab272733 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse colon is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Immunocytochemistry - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Immunocytochemistry - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Immunofluorescent analysis of 100% methanol-fixed PC-12 cells labelling L1CAM with ab272733 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000  dilution (Green). Confocal image showing membranous and cytoplasmic staining in PC-12 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200  dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver tissue labeling L1CAM with ab272733 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: No staining on rat liver (PMID: 22888955) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Immunohistochemistry (Frozen sections) - Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling L1CAM with ab272733 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: No staining on mouse liver (PMID: 22888955) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272733).

  • Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)
    Anti-L1CAM antibody [EPR23338-106] - BSA and Azide free (ab273518)

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