Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (mono methyl K36) antibody [EPR16993] - BSA and Azide free (ab208581)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16993] to Histone H3 (mono methyl K36) - BSA and Azide free
  • Suitable for: ICC, PepArr, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Histone H3 (mono methyl K36) antibody [EPR16993] - BSA and Azide free
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [EPR16993] to Histone H3 (mono methyl K36) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, PepArr, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and NIH/3T3 cell lysates. ICC/IF: HeLa cells.

  • General notes

    Ab208581 is the carrier-free version of ab176920. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab208581 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16993

  • Isotype

    IgG

  • Research areas

Images

  • Peptide Array - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] - BSA and Azide free (ab208581)
    Peptide Array - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] - BSA and Azide free (ab208581)
    This data was developed using ab176920, the same antibody clone in a different buffer formulation.ab176920 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
  • Western blot - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] - BSA and Azide free (ab208581)
    Western blot - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] - BSA and Azide free (ab208581)
    All lanes : Anti-Histone H3 (mono methyl K36) antibody [EPR16993] (ab176920) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 5 seconds


    This data was developed using ab176920, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] - BSA and Azide free (ab208581)
    Immunocytochemistry - Anti-Histone H3 (mono methyl K36) antibody [EPR16993] - BSA and Azide free (ab208581)
    This data was developed using ab176920, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3 (mono methyl K36) with ab176920 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab176920 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Anti-Histone H3 (mono methyl K36) antibody [EPR16993] - BSA and Azide free (ab208581)
    Anti-Histone H3 (mono methyl K36) antibody [EPR16993] - BSA and Azide free (ab208581)

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