Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17974] to p53 DINP1/TP53INP1
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-p53 DINP1/TP53INP1 antibody [EPR17974]
See all p53 DINP1/TP53INP1 primary antibodies
Description

Rabbit monoclonal [EPR17974] to p53 DINP1/TP53INP1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2, SK-BR-3 C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human pancreas lysate. IHC-P: Human colonic adenocarcinoma, mouse kidney and rat kidney tissues. ICC/IF: HepG2 cells.
General notes
This product was previously labelled as p53 DINP1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17974
Isotype

IgG
Research areas

Western blot - Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026)

All lanes : Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026) at 1/10000 dilution

Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 30 seconds

5% NFDM/TBST: Blocking and diluting buffer.
Immunocytochemistry/ Immunofluorescence - Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling p53 DINP1/TP53INP1 with ab202026 at 1/300 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Confocal image showing nuclear staining on HepG2 cells.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab202026 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026)

Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026) at 1/10000 dilution + Human pancreas lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 30 seconds

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026)

All lanes : Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026) at 1/2000 dilution

Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026)

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling p53 DINP1/TP53INP1 with ab202026 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasm and nucleus staining on cancer cells of Human colonic adenocarcinoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling p53 DINP1/TP53INP1 with ab202026 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasm staining on epithelial cells of mouse kidney tubules is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling p53 DINP1/TP53INP1 with ab202026 at 1/150 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasm and weak nucleus staining on epithelial cells of rat kidney tubules is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-p53 DINP1/TP53INP1 antibody [EPR17974] (ab202026)

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