Anti-Malignant fibrous histiocytoma antibody (ab185289)
Key features and details
- Rabbit polyclonal to Malignant fibrous histiocytoma
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Malignant fibrous histiocytoma antibody -
Description
Rabbit polyclonal to Malignant fibrous histiocytoma -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human Malignant fibrous histiocytoma aa 950-1050.
Database link: Q9Y4C4 -
Positive control
- IHC-P:Human rectum tissue; WB: RT-4 and U-251 MG whole cell lysate; ICC: U-2 OS whole cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Malignant fibrous histiocytoma antibody (ab185289)
Immunohistochemical analysis of paraffin-embedded human rectum tissue labeling malignant fibrous histiocytoma in the nucleus and cytoplasm of glandular cells with ab185289 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-Malignant fibrous histiocytoma antibody (ab185289)All lanes : Anti-Malignant fibrous histiocytoma antibody (ab185289) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Predicted band size: 117 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Malignant fibrous histiocytoma antibody (ab185289)Immunocytochemistry analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling malignant fibrous histiocytoma in the nucleoplasm and cytosol with ab185289 at 2 µg/ml.