Anti-KLF5 antibody (ab137676)
Key features and details
- Rabbit polyclonal to KLF5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KLF5 antibody
See all KLF5 primary antibodies -
Description
Rabbit polyclonal to KLF5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseHumanIHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human KLF5 aa 69-346.
Database link: Q13887 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-KLF5 antibody (ab137676) at 1/5000 dilution
Lane 1 : Non-transfected 293T whole cell extract
Lane 2 : Transfected 293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa
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Immunofluorescence analysis of paraformaldehyde-fixed Mouse ESC D3 cells labelling KLF5 using ab137676 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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All lanes : Anti-KLF5 antibody (ab137676) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa
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HeLa cells stained for KLF5 (green) using ab137676 at 1/500 dilution in ICC/IF.
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ab137676 staining KLF5 in nucleus on HeLa xenograft by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody at a dilution of 1/500
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Anti-KLF5 antibody (ab137676) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 51 kDa
10% SDS PAGE