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Cancer Oncoproteins/suppressors Tumor suppressors p53 pathway

Anti-KLF5 antibody (ab137676)

Price and availability

335 040 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-KLF5 antibody (ab137676)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to KLF5
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-KLF5 antibody
    See all KLF5 primary antibodies
  • Description

    Rabbit polyclonal to KLF5
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human KLF5 aa 69-346.
    Database link: Q13887

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Stem Cells
    • Mesenchymal Stem Cells
    • Intracellular
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Cell survival & death
    • Cardiovascular
    • Heart
    • Apoptosis
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Krueppel like factor
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Krueppel like factor

Images

  • Western blot - Anti-KLF5 antibody (ab137676)
    Western blot - Anti-KLF5 antibody (ab137676)
    All lanes : Anti-KLF5 antibody (ab137676) at 1/5000 dilution

    Lane 1 : Non-transfected 293T whole cell extract
    Lane 2 : Transfected 293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676)
    Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676)
    Immunofluorescence analysis of paraformaldehyde-fixed Mouse ESC D3 cells labelling KLF5 using ab137676 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Western blot - Anti-KLF5 antibody (ab137676)
    Western blot - Anti-KLF5 antibody (ab137676)
    All lanes : Anti-KLF5 antibody (ab137676) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676)
    Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676)

    HeLa cells stained for KLF5 (green) using ab137676 at 1/500 dilution in ICC/IF. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (ab137676)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (ab137676)

    ab137676 staining KLF5 in nucleus on HeLa xenograft by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody at a dilution of 1/500

  • Western blot - Anti-KLF5 antibody (ab137676)
    Western blot - Anti-KLF5 antibody (ab137676)
    Anti-KLF5 antibody (ab137676) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 51 kDa



    10% SDS PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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