Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PIG3 antibody (ab64798)

Key features and details

  • Rabbit polyclonal to PIG3
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PIG3 antibody
    See all PIG3 primary antibodies

  • Description

    Rabbit polyclonal to PIG3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human PIG3.

    Read Abcam's proprietary immunogen policy (Peptide available as ab68589.)

  • Positive control

    • Recombinant Human PIG3 protein (ab95857) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Caco2, A549, WI-38, SW480, TE671, Human Lung Tissue, Human Skeletal Muscle Tissue, Human Colon Tissue

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Cow
Chimpanzee
Rhesus monkey
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Target

  • Function

    May be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. Has low NADPH-dependent diamine reductase activity (in vitro).

  • Sequence similarities

    Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
  • Information by UniProt

  • Database links

    • Alternative names

      • p53 induced gene 3 protein antibody
      • p53-induced gene 3 protein antibody
      • Putative quinone oxidoreductase antibody
      • QORX_HUMAN antibody
      • quinone oxidoreductase homolog antibody
      • Quinone oxidoreductase PIG3 antibody
      • TP53I3 antibody
      • Tumor protein p53 inducible protein 3 antibody
      • Tumor protein p53-inducible protein 3 antibody
      see all

    Images

    • Western blot - Anti-PIG3 antibody (ab64798)
      Western blot - Anti-PIG3 antibody (ab64798)
      All lanes : Anti-PIG3 antibody (ab64798) at 1 µg/ml

      Lane 1 : Caco-2 whole cell lysate (ab3950)
      Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
      Lane 3 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
      Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
      Lane 5 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
      Lane 6 : Lung (Human) Tissue Lysate
      Lane 7 : Human skeletal muscle tissue lysate - total protein (ab29330)
      Lane 8 : Human colon tissue lysate - total protein (ab30051)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 35 kDa
      Observed band size: 35 kDa


      Exposure time: 2 minutes

    References (3)

    Publishing research using ab64798? Please let us know so that we can cite the reference in this datasheet.

    ab64798 has been referenced in 3 publications.

    • Mungamuri SK  et al. Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters. Oncogene 0:N/A (2014). PubMed: 25023704
    • Kracikova M  et al. A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ 20:576-88 (2013). PubMed: 23306555
    • Mungamuri SK  et al. p53-mediated heterochromatin reorganization regulates its cell fate decisions. Nat Struct Mol Biol 19:478-84, S1 (2012). WB . PubMed: 22466965

    Images

    • Western blot - Anti-PIG3 antibody (ab64798)
      Western blot - Anti-PIG3 antibody (ab64798)
      All lanes : Anti-PIG3 antibody (ab64798) at 1 µg/ml

      Lane 1 : Caco-2 whole cell lysate (ab3950)
      Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
      Lane 3 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
      Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
      Lane 5 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
      Lane 6 : Lung (Human) Tissue Lysate
      Lane 7 : Human skeletal muscle tissue lysate - total protein (ab29330)
      Lane 8 : Human colon tissue lysate - total protein (ab30051)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 35 kDa
      Observed band size: 35 kDa


      Exposure time: 2 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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