Anti-IP3 receptor antibody (ab97823)
Key features and details
- Rabbit polyclonal to IP3 receptor
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IP3 receptor antibody
See all IP3 receptor primary antibodies -
Description
Rabbit polyclonal to IP3 receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human IP3 receptor aa 2700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab107795) -
Positive control
- This antibody gave a positive signal in HeLa and PC3 Whole Cell Lysates. A band of the expected size was also detected in MALME 3M and Raji Whole Cell Lysates (data not shown). In our hands, the signal was relatively weak in these lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 314 kDa (predicted molecular weight: 314 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 314 kDa (predicted molecular weight: 314 kDa).Target
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Function
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory. -
Sequence similarities
Belongs to the InsP3 receptor family.
Contains 5 MIR domains. -
Domain
The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. -
Post-translational
modificationsPhosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
Phosphorylated on tyrosine residues. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 3708 Human
- Entrez Gene: 16438 Mouse
- Entrez Gene: 25262 Rat
- Omim: 147265 Human
- SwissProt: Q14643 Human
- SwissProt: P11881 Mouse
- SwissProt: P29994 Rat
- Unigene: 567295 Human
see all -
Alternative names
- 4 antibody
- 5-trisphosphate receptor antibody
- 5-trisphosphate receptor type 1 antibody
see all
Images
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Western blot - Anti-IP3 receptor antibody (ab97823)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IP3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SHSY-5Y whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab97823 observed at 314 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab97823 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when IP3 knockout cells were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. Ab97823 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-IP3 receptor antibody (ab97823)All lanes : Anti-IP3 receptor antibody (ab97823) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 314 kDa
Observed band size: 314 kDa
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab97823 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-IP3 receptor antibody (ab97823)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IP3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SHSY-5Y whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab97823 observed at 314 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab97823 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when IP3 knockout cells were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. Ab97823 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-IP3 receptor antibody (ab97823)All lanes : Anti-IP3 receptor antibody (ab97823) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 314 kDa
Observed band size: 314 kDa
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
