Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸

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Anti-IP3 receptor antibody [EPR4536] (ab125076)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4536] to IP3 receptor
Suitable for: WB, IHC-P, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-IP3 receptor antibody [EPR4536]
See all IP3 receptor primary antibodies
Description

Rabbit monoclonal [EPR4536] to IP3 receptor
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human IP3 receptor aa 1-100. The exact sequence is proprietary.
Positive control
- SH-SY5Y, HeLa and HuT78 cell lysates; Human brain tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4536
Isotype

IgG
Research areas

Western blot - Anti-IP3 receptor antibody [EPR4536] (ab125076)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IP3 receptor knockout HAP1 cell lysate (20 µg)
Lane 3: Human brain tissue lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab125076 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab125076 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IP3 receptor knockout samples were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab125076 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4536] (ab125076)

ab125076, at 1/50, staining ITPR1 in paraffin-embedded Human brain tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-IP3 receptor antibody [EPR4536] (ab125076)

All lanes : Anti-IP3 receptor antibody [EPR4536] (ab125076) at 1/1000 dilution

Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HuT78 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 314 kDa
Anti-IP3 receptor antibody [EPR4536] (ab125076)

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