Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IP3 receptor antibody [EPR4537] (ab108517)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4537] to IP3 receptor
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-IP3 receptor antibody [EPR4537]
    See all IP3 receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR4537] to IP3 receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    IP
    Mouse
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat brain, SH-SY5Y, Mouse brain, and HeLa lysates IP: Mouse brain cells; IHC-P: Human and Mouse cerebellum tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: IP3 receptor knockout HAP1 cell lysate (20 µg)
    Lane 3: Human brain tissue lysate (20 µg)
    Lane 4: SH-SY5Y cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab108517 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.


    ab108517 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IP3 receptor knockout samples were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab108517 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    Immunohistochemical analysis of paraffin-embedded Human cerebellum  tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunoprecipitation - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Immunoprecipitation - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    ab108517 (purified) at 1:500 dilution (1.124 µg/ml) immunoprecipitating IP3 receptor in Mouse brain lysate. 
    Lane 1 (input): Mouse brain lysate 10µg
    Lane 2 (+): ab108517 & Mouse brain lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32061 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST.

  • Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    All lanes : Anti-IP3 receptor antibody [EPR4537] (ab108517) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : SH-SY5Y lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : HeLa brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 314 kDa

  • OI-RD Scanning - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    OI-RD Scanning - Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-IP3 receptor antibody [EPR4537] (ab108517)
    Anti-IP3 receptor antibody [EPR4537] (ab108517)

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