Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸ 10 µl 134 016 ₸

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Anti-IP3 receptor antibody [EPR4537] (ab108517)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4537] to IP3 receptor
Suitable for: WB, IHC-P, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-IP3 receptor antibody [EPR4537]
See all IP3 receptor primary antibodies
Description

Rabbit monoclonal [EPR4537] to IP3 receptor
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
IP	Mouse
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat brain, SH-SY5Y, Mouse brain, and HeLa lysates IP: Mouse brain cells; IHC-P: Human and Mouse cerebellum tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 2.10 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4537
Isotype

IgG
Research areas

Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IP3 receptor knockout HAP1 cell lysate (20 µg)
Lane 3: Human brain tissue lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108517 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab108517 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IP3 receptor knockout samples were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab108517 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunoprecipitation - Anti-IP3 receptor antibody [EPR4537] (ab108517)

ab108517 (purified) at 1:500 dilution (1.124 µg/ml) immunoprecipitating IP3 receptor in Mouse brain lysate.
Lane 1 (input): Mouse brain lysate 10µg
Lane 2 (+): ab108517 & Mouse brain lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32061 in HeLa whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST.
Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)

All lanes : Anti-IP3 receptor antibody [EPR4537] (ab108517) at 1/1000 dilution

Lane 1 : Rat brain lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : Mouse brain lysate
Lane 4 : HeLa brain lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 314 kDa
OI-RD Scanning - Anti-IP3 receptor antibody [EPR4537] (ab108517)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-IP3 receptor antibody [EPR4537] (ab108517)

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