Anti-KLF5 antibody (ab230861)
Key features and details
- Rabbit polyclonal to KLF5
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KLF5 antibody
See all KLF5 primary antibodies -
Description
Rabbit polyclonal to KLF5 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human KLF5 aa 341-370 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q13887 -
Positive control
- IHC-P: Human kidney tissue. ICC/IF: U-251 MG cells. WB: CCRF-CEM cell lysate. Flow Cyt: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KLF5 antibody (ab230861)Anti-KLF5 antibody (ab230861) at 1/1000 dilution + CCRF-CEM (Human T cell lymphoblast cell line) cell lysate at 35 µg
Predicted band size: 51 kDa
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Flow Cytometry - Anti-KLF5 antibody (ab230861)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling KLF5 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (ab230861)4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells labeling KLF5 (green) using ab230861 at 1/10 dilution in ICC/IF.
For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody was used (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (ab230861)Formalin-fixed, paraffin-embedded human kidney tissue stained for KLF5 using ab230861 at 1/10 dilution in immunohistochemical analysis.
