Anti-FGFR1 antibody [EPR806Y] - BSA and Azide free (ab271861)
![Anti-FGFR1 antibody [EPR806Y] - BSA and Azide free (ab271861)](https://www.abcam.com/ps/products/271/ab271861/Images/ab271861-1-ab76464356821antifgfr1antibodyepr806yimmunocytochemistryimmunofluorescenceshsy57human.jpg "Anti-FGFR1 antibody [EPR806Y] - BSA and Azide free (ab271861)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR806Y] to FGFR1 - BSA and Azide free
- Suitable for: WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-FGFR1 antibody [EPR806Y] - BSA and Azide free
See all FGFR1 primary antibodies -
Description
Rabbit monoclonal [EPR806Y] to FGFR1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: A-204 cell lysate ICC/IF: SH-SY5Y cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
ab271861 is the carrier-free version of ab76464. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR806Y -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody [EPR806Y] - BSA and Azide free (ab271861)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling FGFR1 with at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in SH-SY5Y cell line is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76464). -
![Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] - BSA and Azide free (ab271861)](https://www.abcam.com/ps/products/271/ab271861/Images/ab271861-2-ab76464339336ab76464IP1.jpg)
Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] - BSA and Azide free (ab271861)ab76464 (purified) at 1/190 immunoprecipitating FGFR1 in 10 μg A-204 (Human muscle rhabdomyosarcoma)whole cell lysate (Lanes 1 and 2, observed at 145 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730) instead of ab76464 in A-204 whole cell lysate. For western blotting, ab76464 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76464).
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![Anti-FGFR1 antibody [EPR806Y] - BSA and Azide free (ab271861)](https://www.abcam.com/ps/products/271/ab271861/Images/ab271861-3-benefits-of-recombinant-antibodies.png)
Anti-FGFR1 antibody [EPR806Y] - BSA and Azide free (ab271861)
