Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR843(N)] to FGFR1 (phospho Y653)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-FGFR1 (phospho Y653) antibody [EPR843(N)]
See all FGFR1 primary antibodies -
Description
Rabbit monoclonal [EPR843(N)] to FGFR1 (phospho Y653) -
Host species
Rabbit -
Specificity
ab173305 only detects FGFR1 phosphorylated at Tyrosine 653. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse -
Immunogen
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Positive control
- WB: NIH/3T3 cell lysate treated with pervanadate. IHC-P: Human glioma tissue.
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General notes
This antibody was developed as part of a collaboration between Epitomics and Ira Daar at the National Cancer Institute, NIH.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR843(N) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-FGFR1 (phospho Y653) antibody [EPR843(N)] (ab173305) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size: 92 kDa
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Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling FGFR1 (phospho Y653) with ab173305 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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