Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (ab206382)
![Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (ab206382)](https://www.abcam.com/ps/products/206/ab206382/Images/ab206382-283260-anti-fgfr1-antibody-epr806y-western-blot.jpg "Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (ab206382)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR806Y] to FGFR1 - Low endotoxin, Azide free
- Suitable for: IP, WB, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free
See all FGFR1 primary antibodies -
Description
Rabbit monoclonal [EPR806Y] to FGFR1 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y cell lysate.
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General notes
ab206382 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR806Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (ab206382)
This WB data was generated using the same anti-FGFR1 antibody clone [EPR806Y] in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (cat# ab76464).
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: FGFR1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: MCF7 whole cell lysate (20 µg)
Lane 4: SH-SY5Y whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab76464 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76464 was shown to specifically react with FGFR1 when FGFR1 knockout samples were used. Wild-type and FGFR1 knockout samples were subjected to SDS-PAGE. ab76464 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/mL and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (ab206382)](https://www.abcam.com/ps/products/206/ab206382/Images/ab206382-341485-ab76464IP1.jpg)
Immunoprecipitation - Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (ab206382)ab76464 (purified) at 1/500 immunoprecipitating FGFR1 in 10 μg A-204 (Human muscle rhabdomyosarcoma)whole cell lysate (Lanes 1 and 2, observed at 145 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730) instead of ab76464 in A-204 whole cell lysate. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76464).
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![Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (ab206382)](https://www.abcam.com/ps/products/206/ab206382/Images/ab206382-4-benefits-of-recombinant-antibodies.png)
Anti-FGFR1 antibody [EPR806Y] - Low endotoxin, Azide free (ab206382)
