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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] - BSA and Azide free (ab240940)

Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] - BSA and Azide free (ab240940)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5193] to Carbonic Anhydrase 1/CA1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] - BSA and Azide free
    See all Carbonic Anhydrase 1/CA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5193] to Carbonic Anhydrase 1/CA1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Zebrafish
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: TF-1, Mouse spleen lysates, Rat brain lysates, Rat spleen lysates, Zebrafish eye lysates, Mouse kidney lysates, HEL, K-562 whole cell lysates Flow Cyt: K-562 Cells IHC-Fr: Zebrafish retina tissue.
  • General notes

    ab240940 is the carrier-free version of ab108367 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240940 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Carbonic Anhydrase I

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5193
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Flow Cytometry - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] - BSA and Azide free (ab240940)
    Flow Cytometry - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] - BSA and Azide free (ab240940)

    Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Carbonic Anhydrase I/CA1 with Purified ab108367 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108367)

  • Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] - BSA and Azide free (ab240940)
    Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] - BSA and Azide free (ab240940)
    All lanes : Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367) at 1/10000 dilution

    Lane 1 : K562 cell lysates
    Lane 2 : TF1 cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 29 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108367).

  • Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] - BSA and Azide free (ab240940)
    Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] - BSA and Azide free (ab240940)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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