Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] - BSA and Azide free (ab240940)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5193] to Carbonic Anhydrase 1/CA1 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] - BSA and Azide free
See all Carbonic Anhydrase 1/CA1 primary antibodies -
Description
Rabbit monoclonal [EPR5193] to Carbonic Anhydrase 1/CA1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Zebrafish -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: TF-1, Mouse spleen lysates, Rat brain lysates, Rat spleen lysates, Zebrafish eye lysates, Mouse kidney lysates, HEL, K-562 whole cell lysates Flow Cyt: K-562 Cells IHC-Fr: Zebrafish retina tissue.
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General notes
ab240940 is the carrier-free version of ab108367 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab240940 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Carbonic Anhydrase I
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5193 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Carbonic Anhydrase I/CA1 with Purified ab108367 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108367) -
All lanes : Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367) at 1/10000 dilution
Lane 1 : K562 cell lysates
Lane 2 : TF1 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108367).
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