Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23232-286] to Carbonic Anhydrase 1/CA1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286]
See all Carbonic Anhydrase 1/CA1 primary antibodies -
Description
Rabbit monoclonal [EPR23232-286] to Carbonic Anhydrase 1/CA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P MouseRatHumanWB MouseRatHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEL, TF-1, Human lung, Human pancreas, Human colon, Mouse spleen, Mouse colon and Rat spleen lysates. IHC-P: Human bone marrow, Human lung, Human colon, Mouse colon and Rat colon tissues. Flow Cyt: HEL cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23232-286 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475) at 1/1000 dilution
Lane 1 : HEL (human erythroleukemia erythroblast) whole cell lysate at 20 µg
Lane 2 : TF-1 (human erythroleukemia erythroblast) whole cell lysate at 20 µg
Lane 3 : Human lung tissue lysate at 20 µg
Lane 4 : Human pancreas tissue lysate at 20 µg
Lane 5 : Human colon tissue lysate at 20 µg
Lane 6 : Mouse spleen tissue lysate 20 ug
Lane 7 : Mouse colon tissue at 20 µg
Lane 8 : Rat spleen tissue at 20 µg
Secondary
Lanes 1-2 & 7-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 3-6 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution
Predicted band size: 28 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 5.5 seconds
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEL (Human Erythroleukemia erythroblast) cells labelling Carbonic Anhydrase 1/CA with ab267475 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat colon (PMID: 21763241) is observed. The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse colon (PMID: 21763241) is observed. The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon (PMID: 21763241). The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red blood cells in human lung is observed. The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human bone marrow. The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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