Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23232-286] to Carbonic Anhydrase 1/CA1
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286]
See all Carbonic Anhydrase 1/CA1 primary antibodies
Description

Rabbit monoclonal [EPR23232-286] to Carbonic Anhydrase 1/CA1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEL, TF-1, Human lung, Human pancreas, Human colon, Mouse spleen, Mouse colon and Rat spleen lysates. IHC-P: Human bone marrow, Human lung, Human colon, Mouse colon and Rat colon tissues. Flow Cyt: HEL cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23232-286
Isotype

IgG
Research areas

Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475)

All lanes : Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475) at 1/1000 dilution

Lane 1 : HEL (human erythroleukemia erythroblast) whole cell lysate at 20 µg
Lane 2 : TF-1 (human erythroleukemia erythroblast) whole cell lysate at 20 µg
Lane 3 : Human lung tissue lysate at 20 µg
Lane 4 : Human pancreas tissue lysate at 20 µg
Lane 5 : Human colon tissue lysate at 20 µg
Lane 6 : Mouse spleen tissue lysate 20 ug
Lane 7 : Mouse colon tissue at 20 µg
Lane 8 : Rat spleen tissue at 20 µg

Secondary
Lanes 1-2 & 7-8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 3-6 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

Predicted band size: 28 kDa
Observed band size: 29 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 5.5 seconds
Flow Cytometry - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEL (Human Erythroleukemia erythroblast) cells labelling Carbonic Anhydrase 1/CA with ab267475 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat colon (PMID: 21763241) is observed. The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse colon (PMID: 21763241) is observed. The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon (PMID: 21763241). The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red blood cells in human lung is observed. The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475)

Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling Carbonic Anhydrase 1/CA with ab267475 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human bone marrow. The section was incubated with ab267475 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (ab267475)

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