Anti-NDUFS8 antibody [EPR11893] - C-terminal (ab180183)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11893] to NDUFS8 - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-NDUFS8 antibody [EPR11893] - C-terminal
See all NDUFS8 primary antibodies -
Description
Rabbit monoclonal [EPR11893] to NDUFS8 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NDUFS8 aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O00217 -
Positive control
- Fetal heart, HepG2, Jurkat and HeLa lysates; Human endometrial carcinoma, Human heart and Human kidney tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11893 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NDUFS8 antibody [EPR11893] - C-terminal (ab180183) at 1/1000 dilution
Lane 1 : Fetal heart lysate
Lane 2 : HepG2 lysate
Lane 3 : Jurkat lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
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Flow Cytometrical analysis of permeabilized HeLa cells labeling NDUFS8 with ab180183 at 1/10 (red) or a rabbit IgG negative (green).
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Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling NDUFS8 with ab180183 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human heart tissue labeling NDUFS8 with ab180183 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue labeling NDUFS8 with ab180183 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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