Picro-Sirius Red Solution ab246832 is intended for use in the histological visualization of muscle and collagen fibers in tissue sections. The stain may be viewed using standard light microscopy or polarized light resulting in birefringence of collagen fibers. Please refer to ab150681 Picro Sirius Red Stain Kit (Connective Tissue Stain) for more information on this solution as it is a component of the kit.

Light Microscopy
Collagen: Red
Muscle Fibers: Yellow
Cytoplasm: Yellow
Polarized Light Microscopy
Collagen: Yellow-Orange and Green Birefringence

Control Tissues: Lung, Uterus, Muscle, Kidney

Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Apply adequate Picro-Sirius Red Solution to completely cover tissue section and incubate for 60 minutes.
3. Rinse slide quickly in two changes of Acetic Acid Solution (0.5%).
4. Rinse slide using absolute alcohol.
5. Dehydrate in 2 changes of absolute alcohol, clear, and mount in synthetic resin.

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.