Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24848-9] to SARS2-CoV-2 Protein 3a - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt, WB

Overview

  • Product name

    Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free
    See all SARS2-CoV-2 Protein 3a primary antibodies

  • Description

    Rabbit monoclonal [EPR24848-9] to SARS2-CoV-2 Protein 3a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: SARS-CoV-2

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T cells transfected with myc-tagged SARS-CoV-2 Protein 3a. IHC-P: 293T cells transfected with myc-tagged SARS-CoV-2 Protein 3a. ICC: 293T cells transfected with myc-tagged SARS-CoV-2 Protein 3a. Flow Cyt: 293T cells transfected with myc-tagged SARS-CoV-2 Protein 3a. IP: 293T cells transfected with myc-tagged SARS-CoV-2 Protein 3a.

  • General notes

    Please Note: At the moment, this product is only available for our customers based in Mainland China.  We are expecting to make his product available to our customers globally in Spring 2021.

    ab280961 is the carrier-free version of ab280953. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280961 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24848-9

  • Isotype

    IgG

Images

  • Western blot - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)
    Western blot - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)
    All lanes : Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] (ab280953) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with SARS-CoV-2 Protein 3a expression vector containing a myc-His-tag®, whole cell lysate
    Lane 3 : HEK-293T transfected with SARS-CoV-1 Protein 3a expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Observed band size: 23 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab280953, the same antibody clone in a different buffer formulation.

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time 5.5 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)

    This data was developed using ab280953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded HEK-293T cells labelling SARS2-CoV-2 Protein 3a with ab280953 at 1/1000 (0.53 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Panel A : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with SARS-CoV-2 Protein 3a expression vector containing a myc-His-tag® Panel B: HEK-293T transfected with SARS-CoV-1 Protein 3a vector containing a myc-His-tag®. Panel C: Incubate HEK-293T transfected with SARS-CoV-2 Protein 3a expression vector containing a myc-His-tag® with secondary antibody only, but not ab280953. Panel D: HEK-293T transfected with control vector containing a myc-His-tag®. Positive staining on (A) HEK-293T transfected with SARS-CoV-2 Protein 3a expression vector. No staining on (B) HEK-293T transfected with a SARS-CoV-1 Protein 3a expression vector, (C) HEK-293T transfected with SARS-CoV-2 Protein 3a expression vector and incubated with secondary antibody only and (D) HEK-293T transfected with empty vector. The section was incubated with ab280953 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)

    This data was developed using ab280953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling SARS2-CoV-2 Protein 3a with ab280953 at 1/1000 (0.53 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). No staining on human lung. The section was incubated with ab280952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)

    This data was developed using ab280953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labelling SARS2-CoV-2 Protein 3a with ab280953 at 1/1000 (0.53 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). No staining on mouse lung. The section was incubated with ab280952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)

    This data was developed using ab280953, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labelling SARS2-CoV-2 Protein 3a with ab280953 at 1/1000 (0.53 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). No staining on rat lung. The section was incubated with ab280952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)
    Immunocytochemistry - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)

    This data was developed using ab280953, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling SARS2-CoV-2 Protein 3a with ab280953 at 1/500 dilution (1.06 μg/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged SARS-CoV-2 Protein 3a expression vector, and no staining in 293T cells transfected with myc-tagged SARS-COV-1 Protein 3a expression vector. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain myc-tag at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)
    Flow Cytometry - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)

    This data was developed using ab280953, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human embryonic kidney) transfected with myc-tagged SARS-CoV-2 Protein 3a expression construct (Right) with ab280953 at 1/500 dilution compared with HEK-293T (human embryonic kidney) transfected with myc-tagged SARS-COV-1 Protein 3a expression construct (Left). 

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077 at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)
    Immunoprecipitation - Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)

    This data was developed using ab280953, the same antibody clone in a different buffer formulation.

    SARS-CoV-2 Protein 3a was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Protein 3a expression vector containing a myc-His-tag®, whole cell lysate 20 µg with ab280953 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280953 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Protein 3a expression vector containing a myc-His-tag®, whole cell lysate 20 μg

    Lane 2: ab280953 IP in HEK-293T transfected with SARS-CoV-2 Protein 3a expression vector containing a myc-His-tag® whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280953 in HEK-293T transfected with SARS-CoV-2 Protein 3a expression vector containing a myc-His-tag® whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

     

  • Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)
    Anti-SARS2-CoV-2 Protein 3a antibody [EPR24848-9] - BSA and Azide free (ab280961)

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