Mouse G-CSF ELISA Kit (CSF3) (ab197743)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.4 pg/ml
- Range: 12.5 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse G-CSF ELISA Kit (CSF3)
See all G-CSF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% RAW 264.7 5 5.7% Inter-assay Sample n Mean SD CV% RAW 264.7 3 11.9% -
Sample type
Cell culture supernatant, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5.4 pg/ml -
Range
12.5 pg/ml - 800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97.2 86.2% - 106% Cell culture media 103.5 98% - 106.5% Cit plasma 105.2 95.1% - 116.9% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse G-CSF ELISA Kit (CSF3) (ab197743) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of G-CSF (CSF3) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse G-CSF (CSF3) with 5.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF induces granulocytes. G-CSF is a monomeric secreted protein.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse G-CSF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse G-CSF Capture Antibody (Lyophilized) 1 vial Mouse G-CSF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Recombinant human G-CSF has been developed by pharmaceutical companies, and since the late 1980's, multiple clinical trials have explored its efficacy in a variety of conditions. -
Alternative names
- C17orf33
- Colony stimulating factor 3 (granulocyte)
- CSF 3
see all -
Database links
- Entrez Gene: 12985 Mouse
- SwissProt: P09920 Mouse
- Unigene: 1238 Mouse
Images
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Other - Mouse G-CSF ELISA Kit (CSF3) (ab197743)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of G-CSF standard curve for Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of G-CSF standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native G-CSF in mouse serum and plasma (citrate) samples.The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native G-CSF in RAW264.7 LPSstimulated cell culture supernatant samples.RAW 264.7 cells were 24 hour serum starved and continued to culture in the presence of 5 µg/mL lipopolysaccharides (LPS) for 48 hours. The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: RAW264.7 supernatant 33%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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LPS-treatment of RAW 264.7 cells stimulates secretion of G-CSF.RAW 264.7 cells were 24 hour serum starved and continued to culture either in the absence or in the presence of 5 µg/mL lipopolysaccharides (LPS) for 48 hours. Various dilutions (as indicated) of the cell culture supernatant samples were analyzed with this kit. Interpolated concentrations of G-CSF adjusted for sample dilution are graphed in pg of G-CSF per mL of supernatant (mean +/- SD, n = 2). Note that G-CSF is not detectable in untreated RAW 264.7 supernatant samples.
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Sandwich ELISA - Mouse G-CSF ELISA Kit (CSF3) (ab197743)To learn more about the advantages of recombinant antibodies see here.
