Anti-Biotin antibody (ab53494)
Key features and details
- Rabbit polyclonal to Biotin
- Suitable for: WB, Competitive ELISA, IP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Biotin antibody
See all Biotin primary antibodies -
Description
Rabbit polyclonal to Biotin -
Host species
Rabbit -
Specificity
Reacts with free biotin and biotinylated antibody and proteins -
Tested applications
Suitable for: WB, Competitive ELISA, IP, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.00
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified with d-Biotin-agarose beads -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Biotin antibody (ab53494) Image courtesy of an anonymous AbReview
RWP1 cells were lysed with Polysomal Lysis
Buffer and diluted in binding buffer (20 mM Hepes
pH 7.6, 150 mM KCl, 3 mM MgCl2, 10% glycerol, 3 mg/ml BSA, 0.2 Triton X-100, 20 µg/ml poly dI-dC, 1 mM DDT, plus protease and RNase inhibitors). Pre-clearing was performed by incubating with protein G-agarose blocked with salmon sperm (1 mg/mL) and mouse IgG (10 µg/mL). After pre-clearing, samples (500 µg) were incubated 4 hours in binding buffer with the DNA-biotinylated probes (2 µg) and antibody against biotin (20 µg/mL) in a final volume of 1 mL. Samples were pulled-down with protein G-agarose, washed with binding buffer supplemented with 0.1%
Tween-20 and analyzed by Western Blot for Suz12.
