Anti-Biotin antibody [Hyb-8] (ab201341)
![Anti-Biotin antibody [Hyb-8] (ab201341)](https://www.abcam.com/ps/products/201/ab201341/Images/ab201341-243025-anti-biotin-antibody-hyb-8-immunohistochemistry.jpg "Anti-Biotin antibody [Hyb-8] (ab201341)")
Key features and details
- Mouse monoclonal [Hyb-8] to Biotin
- Suitable for: Flow Cyt, In situ hybridization, WB, ICC/IF, IHC-P
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-Biotin antibody [Hyb-8]
See all Biotin primary antibodies -
Description
Mouse monoclonal [Hyb-8] to Biotin -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, In situ hybridization, WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Other Immunogen Type corresponding to Biotin. (Biotinylated sheep immunoglobulin).
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Positive control
- Biotinylated proteins in solution or on tissues.
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General notes
ab201341 has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab201341 is purified from Bioreactor Concentrate by Protein A/G column chromatography. -
Clonality
Monoclonal -
Clone number
Hyb-8 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [Hyb-8] (ab201341)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue stained with biotinylated Lambda Light Chain probe followed by ab201341 at 1 µg/ml.
