Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23752-147] to NG2 - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-NG2 antibody [EPR23752-147] - BSA and Azide free
    See all NG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23752-147] to NG2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IHC-Fr,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cerebrum tissue. ICC: Mouse primary neural/glia cells; Rat primary neural/glia cells. Flow cyt: NIH/3T3 and mouse primary neural glia cells.

  • General notes

    ab275041 is the carrier-free version of ab259324. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

    This data was developed using ab259324, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling NG2 with ab259324 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID: 31769737). The section was incubated with ab259324 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

  • Immunocytochemistry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
    Immunocytochemistry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

    This data was developed using ab259324, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling NG2 with ab259324 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary glia cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
    Flow Cytometry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

    This data was developed using ab259324, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Mouse primary neural glia cells cells labelling NG2 with ab259324 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Flow Cytometry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
    Flow Cytometry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

    This data was developed using ab259324, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of N9 (Mouse microglia, Left) / NIH/3T3 (Mouse embryonic fibroblast, Right) cells labelling NG2 with ab259324 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: N9 cells.

    Gated on viable cells.

  • Immunocytochemistry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
    Immunocytochemistry - Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

    This data was developed using ab259324, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labelling NG2 with ab259324 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in rat primary glia cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.  ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain MAP2 at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab259324 at 1/50 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

    -ve control 2: ab11267 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)
    Anti-NG2 antibody [EPR23752-147] - BSA and Azide free (ab275041)

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