Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)
![Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)](https://www.abcam.com/ps/products/108/ab108367/Images/ab108367-333650-anti-carbonic-anhydrase-i-antibody-epr5193-western-blot.jpg "Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5193] to Carbonic Anhydrase 1/CA1
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human, Zebrafish
Overview
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Product name
Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193]
See all Carbonic Anhydrase 1/CA1 primary antibodies -
Description
Rabbit monoclonal [EPR5193] to Carbonic Anhydrase 1/CA1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: TF-1, Mouse spleen lysates, Rat brain lysates, Rat spleen lysates, Zebrafish eye lysates, Mouse kidney lysates, HEL, K-562 whole cell lysates Flow Cyt: K-562 cells IHC-Fr: Zebrafish retina tissue.
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General notes
This product was previously labelled as Carbonic Anhydrase I
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5193 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)All lanes : Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367) at 1/10000 dilution
Lane 1 : TF-1 (Human Erythroleukemia erythroblast) whole cell lysates
Lane 2 : Mouse spleen lysates
Lane 3 : Rat brain lysates
Lane 4 : Rat spleen lysates
Lane 5 : Zebrafish eye lysates
Lane 6 : Mouse kidney lysates
Lane 7 : HEL (Human Erythroleukemia erythroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
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![Flow Cytometry - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)](https://www.abcam.com/ps/products/108/ab108367/Images/ab108367-333723-anti-carbonic-anhydrase-i-antibody-epr5193-flow-cytometry.jpg)
Flow Cytometry - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Carbonic Anhydrase I/CA1 with Purified ab108367 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)](https://www.abcam.com/ps/products/108/ab108367/Images/ab108367-121001-anti-carbonic-anhydrase-i-antibody-epr5193-western-blot.jpg)
Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)All lanes : Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367) at 1/10000 dilution
Lane 1 : K562 cell lysates
Lane 2 : TF1 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa
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![Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)](https://www.abcam.com/ps/products/108/ab108367/Images/ab108367-5-benefits-of-recombinant-antibodies.png)
Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367)
