Antibodies, Proteins, Kits and Reagents for Life Science

237 878 ₸ Product size

100 µl 237 878 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3238] to PKN
Suitable for: WB, Flow Cyt
Reacts with: Human

Product name

Anti-PKN antibody [EPR3238]
See all PKN primary antibodies
Description

Rabbit monoclonal [EPR3238] to PKN
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- SH-SY-5Y, U937 and Jurkat cell lysates
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3238
Isotype

IgG
Research areas

Western blot - Anti-PKN antibody [EPR3238] (ab108973)

All lanes : Anti-PKN antibody [EPR3238] (ab108973) at 1/1000 dilution

Lane 1 : SH-SY-5Y cell lysates
Lane 2 : U937 cell lysates
Lane 3 : Jurkat cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 104 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-PKN antibody [EPR3238] (ab108973)

Flow cytometric analysis of PKN in permeabilized Jurkat cells, using a 1/10 dilution of ab108973 (red) or a rabbit IgG (negative) (green).
Anti-PKN antibody [EPR3238] (ab108973)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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