Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14698] to AZI2 - C-terminal
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AZI2 antibody [EPR14698] - C-terminal
    See all AZI2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14698] to AZI2 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293, NIH 3T3 and Rat kidney lysate; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253)
    Western blot - Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253)
    Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253) at 1/5000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253)
    Immunocytochemistry/ Immunofluorescence - Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253)

    Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling AZI2 with ab192253 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) ab150078 was used as the secondary at 1/500 dilution.  Couunterstain DAPI. 

    Negative controls: anti-AZI2 at 1/100 dilution, Secondary ab Goat anti mouse IgG (Alexa Fluor®488 at 1/200 dilution.

  • Western blot - Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253)
    Western blot - Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253)
    Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253) at 1/1000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa

  • Western blot - Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253)
    Western blot - Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253)
    All lanes : Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253) at 1/1000 dilution

    Lane 1 : Rat kidney lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa

  • Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253)
    Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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