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Anti-RFC1 antibody - C-terminal (ab229689)

Key features and details

  • Rabbit polyclonal to RFC1 - C-terminal
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RFC1 antibody - C-terminal
    See all RFC1 primary antibodies

  • Description

    Rabbit polyclonal to RFC1 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human RFC1 (C terminal). The exact sequence is proprietary.
    Database link: P35251

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HeLa whole cell extract.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RFC1 antibody - C-terminal (ab229689)
    Western blot - Anti-RFC1 antibody - C-terminal (ab229689)
    All lanes : Anti-RFC1 antibody - C-terminal (ab229689) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 128 kDa



    5 % SDS-PAGE gel.

  • Immunoprecipitation - Anti-RFC1 antibody - C-terminal (ab229689)
    Immunoprecipitation - Anti-RFC1 antibody - C-terminal (ab229689)

    RFC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab229689. Western blot was performed from the immunoprecipitate using ab229689. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HeLa whole cell extract.

    Lane 2: ab229689 IP in HeLa whole cell extract.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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