Anti-RFC1 antibody - C-terminal (ab229689)
Key features and details
- Rabbit polyclonal to RFC1 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RFC1 antibody - C-terminal
See all RFC1 primary antibodies -
Description
Rabbit polyclonal to RFC1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment within Human RFC1 (C terminal). The exact sequence is proprietary.
Database link: P35251 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HeLa whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RFC1 antibody - C-terminal (ab229689)All lanes : Anti-RFC1 antibody - C-terminal (ab229689) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 128 kDa5 % SDS-PAGE gel.
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Immunoprecipitation - Anti-RFC1 antibody - C-terminal (ab229689)RFC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab229689. Western blot was performed from the immunoprecipitate using ab229689. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract.
Lane 2: ab229689 IP in HeLa whole cell extract.
