Anti-RFC1 antibody (ab193559)
Key features and details
- Rabbit polyclonal to RFC1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
- Isotype: IgG
Overview
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Product name
Anti-RFC1 antibody
See all RFC1 primary antibodies -
Description
Rabbit polyclonal to RFC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis -
Immunogen
Recombinant fragment corresponding to Human RFC1 aa 100-350.
Database link: P35251 -
Positive control
- U2OS cells transfected with Flag-RFC1; U2OS, NIH3T3, DT40 and INS1 cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescent analysis of PFA-fixed, methanol permeabilized U2OS cells transfected with Flag-RFC1 labeling RFC1 with ab193559 at 1/2000 dilution (green). Red labeling shows Flag tag detection.
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All lanes : Anti-RFC1 antibody (ab193559) at 1/2000 dilution
Lane 1 : U2OS cell lysate
Lane 2 : NIH3T3 cell lysate
Lane 3 : INS1 cell lysate
Lane 4 : DT40 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 128 kDaIncubated with ab193559 overnight at 4°C in 5% milk TBST (Tween= 0.5%).
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All lanes : Anti-RFC1 antibody (ab193559) at 1/2000 dilution
Lane 1 : Xenopus laevis crude egg extracts
Lane 2 : U2OS whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 128 kDa