Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p73 (phospho Y99) antibody [EPR21751] - BSA and Azide free (ab230887)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21751] to p73 (phospho Y99) - BSA and Azide free
  • Suitable for: WB, Dot blot, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-p73 (phospho Y99) antibody [EPR21751] - BSA and Azide free
    See all p73 primary antibodies

  • Description

    Rabbit monoclonal [EPR21751] to p73 (phospho Y99) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-1376 treated with 1 mM pervanadate for 30 minutes, whole cell lysate.

  • General notes

    Ab230887 is the carrier-free version of ab218625. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab230887 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p73 (phospho Y99) antibody [EPR21751] - BSA and Azide free (ab230887)
    Western blot - Anti-p73 (phospho Y99) antibody [EPR21751] - BSA and Azide free (ab230887)
    All lanes : Anti-p73 (phospho Y99) antibody [EPR21751] - BSA and Azide free (ab230887) at 1/1000 dilution

    Lane 1 : Untreated HT-1376 (human urinary bladder carcinoma cell line) whole cell lysate
    Lane 2 : HT-1376 treated with 1 mM pervanadate for 30 minutes, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 69 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

    The molecular mass observed is consistent with the literature (PMID:11466610).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218625).

  • Immunoprecipitation - Anti-p73 (phospho Y99) antibody [EPR21751] - BSA and Azide free (ab230887)
    Immunoprecipitation - Anti-p73 (phospho Y99) antibody [EPR21751] - BSA and Azide free (ab230887)

    p73 (phospho Y99) was immunoprecipitated from 0.35 mg of HT-1376 (human urinary bladder carcinoma cell line) treated with 1mM Pervanadate for 30 min whole cell lysate with ab218625 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218625 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.

    Lane 1: HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate 10 μg (input).

    Lane 2: ab218625 IP in HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218625 in HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218625).

  • Dot Blot - Anti-p73 (phospho Y99) antibody [EPR21751] - BSA and Azide free (ab230887)
    Dot Blot - Anti-p73 (phospho Y99) antibody [EPR21751] - BSA and Azide free (ab230887)

    Dot blot analysis of p73 (phospho Y99) with ab218625 at 1/1000 dilution.

    Lane 1: p73 (phospho Y99) peptide.

    Lane 2: p73 non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218625).

  • Anti-p73 (phospho Y99) antibody [EPR21751] - BSA and Azide free (ab230887)
    Anti-p73 (phospho Y99) antibody [EPR21751] - BSA and Azide free (ab230887)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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