Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Prohibitin antibody [PHB/1882] - BSA and Azide free (ab269775)

Key features and details

Mouse monoclonal [PHB/1882] to Prohibitin - BSA and Azide free
Suitable for: WB, IHC-P
Reacts with: Human
Isotype: IgG1

Product name

Anti-Prohibitin antibody [PHB/1882] - BSA and Azide free
See all Prohibitin primary antibodies
Description

Mouse monoclonal [PHB/1882] to Prohibitin - BSA and Azide free
Host species

Mouse

Tested Applications & Species

Application	Species
IHC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human Prohibitin aa 167-261. The exact sequence is proprietary.
Database link: P35232
Positive control
- IHC-P: Human liver tissue. WB: Rmoas, HEK293, K562, Jurkat, HepG2, A549, K562, A431 and MCF7 cell lysate; Human liver tissue lysate.
General notes

ab269775 is a carrier free version of ab224657. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from bioreactor concentrate.
Clonality

Monoclonal
Clone number

PHB/1882
Isotype

IgG1
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prohibitin antibody (ab269775)

Formalin-fixed, paraffin-embedded human liver tissue stained for Prohibitin with ab224657 at 2 µg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224657).
Western blot - Anti-Prohibitin antibody [PHB/1882] - BSA and Azide free (ab269775)

All lanes : Anti-Prohibitin antibody [PHB/1882] - BSA and Azide free (ab269775) at 1 µg/ml

Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Predicted band size: 30 kDa

Western blot analysis labeling Prohibitin with ab269775 at 1 ug/ml.
Western blot - Anti-Prohibitin antibody [PHB/1882] - BSA and Azide free (ab269775)

Anti-Prohibitin antibody [PHB/1882] - BSA and Azide free (ab269775) at 1 µg/ml + Human liver tissue lysate

Predicted band size: 30 kDa

Western blot analysis labeling Prohibitin with ab269775 at 1 ug/ml.
Western blot - Anti-Prohibitin antibody [PHB/1882] - BSA and Azide free (ab269775)

All lanes : Anti-Prohibitin antibody [PHB/1882] - BSA and Azide free (ab269775) at 1 µg/ml

Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 30 kDa

Western blot analysis labeling Prohibitin with ab269775 at 1 ug/ml.

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