Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)
![Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)](https://www.abcam.com/ps/products/218/ab218625/Images/ab218625-299057-anti-p73-phospho-y99-antibody-epr21751-western-blot.jpg "Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21751] to p73 (phospho Y99)
- Suitable for: Dot blot, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-p73 (phospho Y99) antibody [EPR21751]
See all p73 primary antibodies -
Description
Rabbit monoclonal [EPR21751] to p73 (phospho Y99) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HT-1376 treated with 1 mM pervanadate for 30 minutes, whole cell lysate. IP: HT-1376 treated with 1 mM pervanadate for 30 minutes, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21751 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)All lanes : Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625) at 1/1000 dilution
Lane 1 : Untreated HT-1376 (human urinary bladder carcinoma cell line) whole cell lysate
Lane 2 : HT-1376 treated with 1 mM pervanadate for 30 minutes, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with the literature (PMID:11466610).
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![Immunoprecipitation - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)](https://www.abcam.com/ps/products/218/ab218625/Images/ab218625-299058-anti-p73-phospho-y99-antibody-epr21751-immunoprecipitation.jpg)
Immunoprecipitation - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)p73 (phospho Y99) was immunoprecipitated from 0.35 mg of HT-1376 (human urinary bladder carcinoma cell line) treated with 1mM Pervanadate for 30 min whole cell lysate with ab218625 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218625 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.
Lane 1: HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate 10 μg (input).
Lane 2: ab218625 IP in HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218625 in HT-1376 treated with 1mM Pervanadate for 30 min whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Dot Blot - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)](https://www.abcam.com/ps/products/218/ab218625/Images/ab218625-299056-anti-p73-phospho-y99-antibody-epr21751-dot-blot.jpg)
Dot Blot - Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)Dot blot analysis of p73 (phospho Y99) with ab218625 at 1/1000 dilution.
Lane 1: p73 (phospho Y99) peptide.
Lane 2: p73 non-phospho peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)](https://www.abcam.com/ps/products/218/ab218625/Images/ab218625-4-benefits-of-recombinant-antibodies.png)
Anti-p73 (phospho Y99) antibody [EPR21751] (ab218625)
