Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23590-68] to BAFF-R - BSA and Azide free
  • Suitable for: Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free
    See all BAFF-R primary antibodies

  • Description

    Rabbit monoclonal [EPR23590-68] to BAFF-R - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Human peripheral blood mononuclear cells. IP: Human spleen tissue lysate.

  • General notes

    ab273572 is the carrier-free version of ab273569. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23590-68

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)
    Immunoprecipitation - Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)

    BAFF-R was immunoprecipitated from 0.35 mg Human spleen tissue lysate  with ab273569 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273569 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human spleen tissue lysate 10 ug

    Lane 2: ab273569 IP in Human spleen tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273569 in Human spleen tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 37 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID:28249164 ).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273569).

  • Flow Cytometry - Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)
    Flow Cytometry - Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling BAFF-R with ab273569 at 1/400 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab273569 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273569).

  • Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)
    Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)

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