Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)
![Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)](https://www.abcam.com/ps/products/273/ab273572/Images/ab273572-2-anti-baffr-antibody-epr2359068-immunoprecipitation-human-spleen-tissue-lysate-10-ug.jpg "Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23590-68] to BAFF-R - BSA and Azide free
- Suitable for: Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free
See all BAFF-R primary antibodies -
Description
Rabbit monoclonal [EPR23590-68] to BAFF-R - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Human peripheral blood mononuclear cells. IP: Human spleen tissue lysate.
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General notes
ab273572 is the carrier-free version of ab273569. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23590-68 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)
BAFF-R was immunoprecipitated from 0.35 mg Human spleen tissue lysate with ab273569 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273569 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human spleen tissue lysate 10 ug
Lane 2: ab273569 IP in Human spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273569 in Human spleen tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 37 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID:28249164 ).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273569).
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![Flow Cytometry - Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)](https://www.abcam.com/ps/products/273/ab273572/Images/ab273572-1-anti-baffr-antibody-epr2359068-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry - Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling BAFF-R with ab273569 at 1/400 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab273569 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273569).
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![Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)](https://www.abcam.com/ps/products/273/ab273572/Images/ab273572-3-benefits-of-recombinant-antibodies.png)
Anti-BAFF-R antibody [EPR23590-68] - BSA and Azide free (ab273572)
