Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [74.1] to Cytoplasmic Dynein Intermediate chain - BSA and Azide free
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free
    See all Cytoplasmic Dynein Intermediate chain primary antibodies

  • Description

    Mouse monoclonal [74.1] to Cytoplasmic Dynein Intermediate chain - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-87 MG, SH-SY5Y, MDA-MB-231, PC-12, C6 and RAW264.7 whole cell lysates. Flow Cyt: HeLa and C6 cells. IP: U-87 MG whole cell lysate. Rat brain tissue lysate.

  • General notes

    ab255981 is the carrier-free version of ab23905. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255981 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    74.1

  • Isotype

    IgG2b

Images

  • Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)
    Western blot - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)
    All lanes : Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] (ab23905) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
    Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
    Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
    Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 73 kDa



    This data was developed using ab23905, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: lane 1-3: 37 seconds; lane 4-6: 81 seconds.

  • Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)
    Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)

    This data was developed using ab23905 the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of C6 (Rat glial tumor glial cell) cells labelling Cytoplasmic Dynein Intermediate chain with ab23905 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)
    Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)

    This data was developed using ab23905 the same antibody clone in a different buffer formulation.

    Cytoplasmic Dynein Intermediate chain was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 10 µg with ab23905 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab23905 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.

    Lane 1: U-87 MG whole cell lysate 10 µg

    Lane 2: ab23905 IP in U-87 MG whole cell lysate

    Lane 3: Mouse monoclonal IgG instead of ab23905 in U-87 MG whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

     

  • Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)
    Flow Cytometry - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)

    This data was developed using ab23905 the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Cytoplasmic Dynein Intermediate chain with ab23905 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)
    Immunoprecipitation - Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)

    This data was developed using ab23905 the same antibody clone in a different buffer formulation.

    Cytoplasmic Dynein Intermediate chain was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 µg with ab23905 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab23905 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.

    Lane 1: Rat brain tissue lysate 10 µg

    Lane 2: ab23905 IP in Rat brain tissue lysate

    Lane 3: Mouse monoclonal IgG instead of ab23905 in rat brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

     

  • Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)
    Anti-Cytoplasmic Dynein Intermediate chain antibody [74.1] - BSA and Azide free (ab255981)

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