Human LBP ELISA Kit (LPS Binding Protein) (ab279407)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.79 pg/ml
- Range: 12.5 pg/ml - 800 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human LBP ELISA Kit (LPS Binding Protein)
See all LBP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 4.4% Inter-assay Sample n Mean SD CV% serum 3 6% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6.79 pg/ml -
Range
12.5 pg/ml - 800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 106 97% - 112% Cell culture media 90 86% - 93% Hep Plasma 100 93% - 108% EDTA Plasma 95 93% - 97% Cit plasma 94 88% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human LBP ELISA kit (ab279407) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human LBP protein in human serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LBP with 6.79 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
LBP (LPS Binding Protein) is an acute phase protein involved in the cellular response to gram-negative bacterial infections. LBP binds to LPS protein expressed on gram-negative bacteria and elicits a response by engaging with pattern recognition receptors, CD14 and TLR4. LBP is essential for the initial response to gram-negative bacterial infection but not for the downstream stages.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human LBP (LPS Binding Protein) Capture Antibody 1 x 600µl 10X Human LBP (LPS Binding Protein) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human LBP (LPS Binding Protein) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. -
Sequence similarities
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BPI fold containing family D, member 2
- Bpifd2
- LBP
see all -
Database links
- Entrez Gene: 3929 Human
- Omim: 151990 Human
- SwissProt: P18428 Human
- Unigene: 154078 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of human LBP standard curve in Sample Diluent NS.The LBP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. The LBP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native LBP in human serum and plasma samples.The concentrations of LBP were measured in duplicates, interpolated from the LBP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 4,000, plasma (citrate) 1: 2,000, plasma (heparin) 1: 2,000, and plasma (EDTA) 1: 2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LBP concentration was determined to be 2.00 µg/mL in serum, 1.39 µg/mL in plasma (citrate), 1.01 µg/mL in plasma (heparin), and 1.50 µg/ml in plasma (EDTA).
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LBP concentration was determined to be 3.11 µg/mL with a range of 0.94 – 4.60 µg/ml.