Transketolase Activity Assay Kit (Fluorometric) (ab273310)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell Lysate, Purified protein, Tissue Lysate
- Sensitivity: 5 µU
Overview
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Product name
Transketolase Activity Assay Kit (Fluorometric)
See all Transketolase kits -
Detection method
Fluorescent -
Sample type
Cell Lysate, Purified protein, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
5 µU -
Assay duration
Multiple steps standard assay -
Product overview
Transketolase Activity Assay Kit (Fluorometric) (ab273310) is a simple, plate-based fluorometric assay for measuring Transketolase (EC 2.2.1.1) TKT activity in biological samples. In this assay, TKT transfers a two-carbon group from a donor keto sugar to an acceptor aldose sugar. The product formed converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction in the presence of a developer and an enzyme mix.
The assay can detect as low as 5 µU of TKT activity in biological samples.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Glyceraldehyde 3-phosphate Standard (Lyophilized) 1 vial TKT Assay Buffer 1 x 35ml TKT Developer (Lyophilized) 1 vial TKT Enzyme Mix (Lyophilized) 1 vial TKT Positive Control (Lyophilized) 1 vial TKT Probe 1 x 400µl TKT Reconstitution Buffer 1 x 200µl TKT Substrate Mix (Lyophilized) 1 vial -
Research areas
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Cellular localization
Cytosol, nuclear, peroxisome, extracellular vesicular exosome -
Alternative names
- epididymis luminal protein 107
- HEL107
- TK
see all
Images
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Gyceraldehyde 3-phosphate standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Enzyme kinetics using rat liver lysate and J774A cell lysate.Example data.
Enzyme kinetics using rat liver lysate (1.2 µg protein/well) and J774A cell lysate (2 µg protein/well).
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TKT specific activity in rat liver lysate and J774A cells.Example data.
Experiments were conducted according to kit protocol.