Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI1A11] to PGM3
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Mouse, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-PGM3 antibody [OTI1A11]
See all PGM3 primary antibodies
Description

Mouse monoclonal [OTI1A11] to PGM3
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human PGM3. Produced in HKE293T cells (NP_056414).
Database link: O95394
Positive control
- WB: HEK-293T cells transfected with pCMV6-ENTRY PGM3 cDNA; HepG2, HeLa, SVT2, COS-7 and MCF7 cell lysates. IHC-P: Human Kidney, Human liver, Human pancreas, Adenocarcinoma of Human endometrium, Human prostate and Carcinoma of Human prostate tissues. Flow: PGM3 transfected HEK-293T cells
General notes

The clone number has been updated from 1A11 to OTI1A11, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography
Clonality

Monoclonal
Clone number

OTI1A11
Isotype

IgG1
Research areas

Western blot - Anti-PGM3 antibody [OTI1A11] (ab128094)

All lanes : Anti-PGM3 antibody [OTI1A11] (ab128094) at 1/500 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY PGM3 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 60 kDa
Western blot - Anti-PGM3 antibody [OTI1A11] (ab128094)

All lanes : Anti-PGM3 antibody [OTI1A11] (ab128094) at 1/500 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 7 : MDCK cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate

Lysates/proteins at 35 µg per lane.

Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

Paraffin-embedded human kidney tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

Paraffin-embedded human liver tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

Paraffin-embedded human pancreas tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

Paraffin-embedded human prostate tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (ab128094)

Paraffin-embedded human prosate carcinoma tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
Flow Cytometry - Anti-PGM3 antibody [OTI1A11] (ab128094)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) using ab128094 at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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