Anti-SUCLA2 antibody (ab190845)
Key features and details
- Rabbit polyclonal to SUCLA2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SUCLA2 antibody
See all SUCLA2 primary antibodies -
Description
Rabbit polyclonal to SUCLA2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Mitochondrial Heart ; Mouse Heart; Mouse Liver as well as both HepG2 and MOLT4 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentrationConcentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab190845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/250. Detects a band of approximately 45 kDa (predicted molecular weight: 50 kDa). Target
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Function
Catalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA. -
Tissue specificity
Widely expressed. Not expressed in liver and lung. -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. -
Involvement in disease
Mitochondrial DNA depletion syndrome 5 -
Sequence similarities
Belongs to the succinate/malate CoA ligase beta subunit family.
Contains 1 ATP-grasp domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 511090 Cow
- Entrez Gene: 8803 Human
- Entrez Gene: 20916 Mouse
- Entrez Gene: 361071 Rat
- Omim: 603921 Human
- SwissProt: Q148D5 Cow
- SwissProt: Q9P2R7 Human
- SwissProt: Q9Z2I9 Mouse
see all -
Alternative names
- A BETA antibody
- A SCS antibody
- ATP specific succinyl CoA synthetase subunit beta antibody
see all
Images
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All lanes : Anti-SUCLA2 antibody (ab190845) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human Heart Mitochondrial Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa (possible non-specific binding)
Exposure time: 30 secondsThe band observed at 45 kDa could potentially be a cleaved form of SUCLA2 due to the presence of 52 amino acid Transit peptide.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab190845 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab190845 has not yet been referenced specifically in any publications.
Images
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All lanes : Anti-SUCLA2 antibody (ab190845) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human Heart Mitochondrial Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa (possible non-specific binding)
Exposure time: 30 secondsThe band observed at 45 kDa could potentially be a cleaved form of SUCLA2 due to the presence of 52 amino acid Transit peptide.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab190845 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.