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Signal Transduction Metabolism Energy Metabolism

Anti-SUCLA2 antibody (ab190845)

Price and availability

274 732 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-SUCLA2 antibody (ab190845)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SUCLA2
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SUCLA2 antibody
    See all SUCLA2 primary antibodies
  • Description

    Rabbit polyclonal to SUCLA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Heart; Human Mitochondrial Heart ; Mouse Heart; Mouse Liver as well as both HepG2 and MOLT4 whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Neuroscience
    • Sensory System
    • Auditory system
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab190845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 45 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Catalyzes the ATP-dependent ligation of succinate and CoA to form succinyl-CoA.
  • Tissue specificity

    Widely expressed. Not expressed in liver and lung.
  • Pathway

    Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
  • Involvement in disease

    Mitochondrial DNA depletion syndrome 5
  • Sequence similarities

    Belongs to the succinate/malate CoA ligase beta subunit family.
    Contains 1 ATP-grasp domain.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession Q9P2R7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 511090 Cow
    • Entrez Gene: 8803 Human
    • Entrez Gene: 20916 Mouse
    • Entrez Gene: 361071 Rat
    • Omim: 603921 Human
    • SwissProt: Q148D5 Cow
    • SwissProt: Q9P2R7 Human
    • SwissProt: Q9Z2I9 Mouse
    • SwissProt: O97580 Pig
    • Unigene: 743361 Human
    • Unigene: 38951 Mouse
    see all
  • Alternative names

    • A BETA antibody
    • A SCS antibody
    • ATP specific succinyl CoA synthetase subunit beta antibody
    • ATP specific succinyl CoA synthetase, beta subunit antibody
    • ATP-specific succinyl-CoA synthetase subunit beta antibody
    • Mitochondrial succinyl CoA ligase [ADP forming] subunit beta antibody
    • MTDPS5 antibody
    • Renal carcinoma antigen NY-REN-39 antibody
    • Renal carcinoma antigen NYREN39 antibody
    • SCS betaA antibody
    • SCS-betaA antibody
    • SUCB1_HUMAN antibody
    • Succinate CoA ligase (ADP forming) antibody
    • Succinate CoA ligase [ADP forming] subunit beta, mitochondrial; succinyl CoA ligase [ADP forming] subunit beta, mitochondrial antibody
    • Succinate CoA ligase ADP forming beta subunit antibody
    • Succinate CoA ligase beta subunit antibody
    • Succinyl CoA ligase [ADP-forming] subunit beta, mitochondrial antibody
    • Succinyl CoA synthetase beta A chain antibody
    • Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial antibody
    • Succinyl-CoA synthetase beta-A chain antibody
    • SUCLA2 antibody
    see all

Images

  • Western blot - Anti-SUCLA2 antibody (ab190845)
    Western blot - Anti-SUCLA2 antibody (ab190845)
    All lanes : Anti-SUCLA2 antibody (ab190845) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human Heart Mitochondrial Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Liver (Mouse) Tissue Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 16 kDa (possible non-specific binding)


    Exposure time: 30 seconds


    The band observed at 45 kDa could potentially be a cleaved form of SUCLA2 due to the presence of 52 amino acid Transit peptide.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab190845 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab190845? Please let us know so that we can cite the reference in this datasheet.

    ab190845 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-SUCLA2 antibody (ab190845)
      Western blot - Anti-SUCLA2 antibody (ab190845)
      All lanes : Anti-SUCLA2 antibody (ab190845) at 1 µg/ml

      Lane 1 : Human heart tissue lysate - total protein (ab29431)
      Lane 2 : Human Heart Mitochondrial Lysate
      Lane 3 : Heart (Mouse) Tissue Lysate
      Lane 4 : Liver (Mouse) Tissue Lysate
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 50 kDa
      Observed band size: 45 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 16 kDa (possible non-specific binding)


      Exposure time: 30 seconds


      The band observed at 45 kDa could potentially be a cleaved form of SUCLA2 due to the presence of 52 amino acid Transit peptide.

      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab190845 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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