Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-SUCLA2 antibody [EPR14925] (ab183513)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14925] to SUCLA2
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Reacts with: Human

Product name

Anti-SUCLA2 antibody [EPR14925]
See all SUCLA2 primary antibodies
Description

Rabbit monoclonal [EPR14925] to SUCLA2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal liver, 293, HepG2 and Molt4 cell lysates, Human breast tissue, HepG2 and 293 cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14925
Isotype

IgG
Research areas

Western blot - Anti-SUCLA2 antibody [EPR14925] (ab183513)

All lanes : Anti-SUCLA2 antibody [EPR14925] (ab183513) at 1/50000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Molt4 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Immunoprecipitation - Anti-SUCLA2 antibody [EPR14925] (ab183513)

Western blot analysis of HepG2 cell lysate immunoprecipitated using ab183513 at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.
Flow Cytometry - Anti-SUCLA2 antibody [EPR14925] (ab183513)

Flow cytometry analysis of 2% paraformaldehyde fixed 293 cells labeling SUCLA2 using ab183513 at a 1/100 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-SUCLA2 antibody [EPR14925] (ab183513)

Immunofluorescent staining of 4% paraformaldehyde fixed 293 cells, labeling SUCLA2 using ab183513 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLA2 antibody [EPR14925] (ab183513)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SUCLA2 using ab183513 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-SUCLA2 antibody [EPR14925] (ab183513)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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