Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SUCLA2 antibody [EPR14925] (ab183513)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14925] to SUCLA2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-SUCLA2 antibody [EPR14925]
    See all SUCLA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14925] to SUCLA2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, 293, HepG2 and Molt4 cell lysates, Human breast tissue, HepG2 and 293 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SUCLA2 antibody [EPR14925] (ab183513)
    Western blot - Anti-SUCLA2 antibody [EPR14925] (ab183513)
    All lanes : Anti-SUCLA2 antibody [EPR14925] (ab183513) at 1/50000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Molt4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa

  • Immunoprecipitation - Anti-SUCLA2 antibody [EPR14925] (ab183513)
    Immunoprecipitation - Anti-SUCLA2 antibody [EPR14925] (ab183513)

    Western blot analysis of HepG2 cell lysate immunoprecipitated using ab183513 at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

  • Flow Cytometry - Anti-SUCLA2 antibody [EPR14925] (ab183513)
    Flow Cytometry - Anti-SUCLA2 antibody [EPR14925] (ab183513)

    Flow cytometry analysis of 2% paraformaldehyde fixed 293 cells labeling SUCLA2 using ab183513 at a 1/100 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-SUCLA2 antibody [EPR14925] (ab183513)
    Immunocytochemistry/ Immunofluorescence - Anti-SUCLA2 antibody [EPR14925] (ab183513)

    Immunofluorescent staining of 4% paraformaldehyde fixed 293 cells, labeling SUCLA2 using ab183513 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLA2 antibody [EPR14925] (ab183513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUCLA2 antibody [EPR14925] (ab183513)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SUCLA2 using ab183513 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-SUCLA2 antibody [EPR14925] (ab183513)
    Anti-SUCLA2 antibody [EPR14925] (ab183513)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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