Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MUC2 antibody [EPR23479-47] (ab272692)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23479-47] to MUC2
  • Suitable for: IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MUC2 antibody [EPR23479-47]
    See all MUC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23479-47] to MUC2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human colon cancer tissue lysate. Mouse small intestine tissue lysate. Rat small intestine and colon tissue lysate. IHC-P: Human, mouse and rat colon tissue. Human bladder cancer tissue. IHC-Fr: Mouse and rat colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat colon tissue labeling MUC2 with ab272692 at 1/500 dilution followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution (Green). Positive staining on rat colon is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling MUC2 with ab272692 at 1/500 dilution followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution (Green). Positive staining on mouse colon is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Western blot - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    All lanes : Anti-MUC2 antibody [EPR23479-47] (ab272692) at 1/1000 dilution

    Lane 1 : Human colon cancer tissue lysate
    Lane 2 : Mouse small intestine tissue lysate
    Lane 3 : Rat small intestine tissue lysate
    Lane 4 : Rat colon tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 540 kDa
    Observed band size: 140-170 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    MUC2 is highly glycosylated.

    The C-terminal fragment alpha-MUC2C2 (140-170 kDa) is observed. The molecular weight is consistent with what have been described in literature (PMID: 12582180; 16754877).  Exposure time: 3 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on rat colon is observed. The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse colon is observed. The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human bladder cancer (PMID: 25197366). The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human colon (PMID: 28693267). The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-MUC2 antibody [EPR23479-47] (ab272692)
    Anti-MUC2 antibody [EPR23479-47] (ab272692)

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