Macrophage Polarization Panel - Human WB (ab278181)
Overview
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Product name
Macrophage Polarization Panel - Human WB -
Species reactivity
Reacts with: Human -
Product overview
Macrophage Polarization Panel - Human WB contains multiple trial-sized versions of anti-human antibody clones against iNOS, CD11b, Liver Arginase, CD163, CD68, CSF-1-R, TIM 3 and Mannose Receptor specifically selected for high performance in various applications. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [EPR16635] to iNOS (20 μL) ab178945
- Rabbit monoclonal [EPR1344] to CD11b (20 μL) ab133357
- Rabbit monoclonal [EPR6672(B)] to Liver Arginase (20 μL) ab133543
- Rabbit monoclonal [EPR19518] to CD163 (20 μL) ab182422
- Rabbit monoclonal [EPR20545] to CD68 (20 μL) ab213363
- Rabbit monoclonal [EPR20754] to CSF-1-R (20 μL) ab229188
- Rabbit monoclonal [EPR22241] to TIM 3 (20 μL) ab241332
- Rabbit monoclonal [EPR22489-7] to Mannose Receptor (20 μL) ab252921
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Please refer to the ‘Associated products’ section below.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Please refer to the ‘Associated products’ section below.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit ab133357 - Anti-CD11b antibody [EPR1344] 2 x 10µl ab182422 - Anti-CD163 antibody [EPR19518] 2 x 10µl ab213363 - Anti-CD68 antibody [EPR20545] 2 x 10µl ab229188 - Anti-CSF-1-R antibody [EPR20754] 2 x 10µl ab178945 - Anti-iNOS antibody [EPR16635] 2 x 10µl ab133543 - Anti-Liver Arginase antibody [EPR6672(B)] 2 x 10µl ab252921 - Anti-Mannose Receptor antibody [EPR22489-7] 2 x 10µl ab241332 - Anti-TIM 3 antibody [EPR22241] 2 x 10µl -
Research areas
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Cellular localization
CD11b: Membrane. CD68: Cell membrane and Endosome membrane. Lysosome membrane. Liver Arginase: Cytoplasm. CD163: Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells. Mannose Receptor: Membrane. TIM 3: Membrane. Cell junction. Localizes to the immunological synapse between CD8+ T-cells and target cells. CSF-1-R: Membrane. -
Database links
- Entrez Gene: 1436 Human
- Entrez Gene: 3684 Human
- Entrez Gene: 383 Human
- Entrez Gene: 4360 Human
- Entrez Gene: 4843 Human
- Entrez Gene: 84868 Human
- Entrez Gene: 9332 Human
- Entrez Gene: 968 Human
see all
Images
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Western blot - Macrophage polarization panel - human WB (ab278181)
All lanes: Anti-TIM 3 antibody [EPR22241] (ab241332) at 1/1000 dilution.
Lane 1: Daudi (human Burkitt's lymphoma cell line) whole cell lysate, 20 ug
Lane 2: Daudi whole cell lysate (treated with PNGase F), 20 mg/ml
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate, 20 ug
Lane 5: RAW 264.7 whole cell lysate (treated with PNGase F), 20 ugSecondary (all lanes): ab97051 at 1/20000 dilution.
Predicted MW: 33 kDa.
Observed MW: 40 kDa, 50-60kDa glycosylated form
Blocking and dilution buffer: 5% NFDM/TBST.
Lanes 3 & 4 were developed using a higher sensitivity ECL substrate.
Exposure: 70seconds
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Western blot - Macrophage polarization panel - human WB (ab278181)All lanes: Anti-CSF-1-R antibody [EPR20754] (ab229188) at 1/1000 dilution.
Lane 1: Human placenta tissue lysate, 10 ug
Lane 2: Human tonsil tissue lysate, 10 ug
Secondary (all lanes): ab131366 at 1/1000 dilution.
Predicted MW: 108 kDa.
Observed MW: 108 kDa.
Blocking/dilution buffer: 5% NFDM/TBST.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
Exposure: 3minute
ECL
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Western blot - Macrophage polarization panel - human WB (ab278181)All lanes: Anti-CD11b antibody [EPR1344] (ab133357) at 1/10000 dilution.
Lane 1: RAW264.7 cell lysate, 20 ug
Lane 2: Mouse spleen tissue lysate, 20 ug
Secondary (all lanes): HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted MW: 127 kDa
Observed MW: 170kDa
Dilution buffer: 5% NFDM/TBSTBlocking buffer: 5% NFDM/TBST -
Western blot - Macrophage polarization panel - human WB (ab278181)All lanes: Anti-iNOS antibody [EPR16635] (ab178945) at 1/500 dilution.
Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates, 15 ug
Lane 2: HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 10ug/ml lipopolysaccharides for 6 hours whole cell lysates, 15 ug
Lane 3: THP-1 (Human monocytic leukemia monocyte) whole cell lysates, 15 ug
Lane 4: THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml lipopolysaccharides for 3 hours whole cell lysates, 15 ugSecondary (all lanes): ab97051 at 1/200000 dilution.
Predicted MW: 131 kDa.
Exposure: 3minute
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Western blot - Macrophage polarization panel - human WB (ab278181) -
Western blot - Macrophage polarization panel - human WB (ab278181)All lanes: Anti-CD163 antibody [EPR19518] (ab182422) at 1/1000 dilution.
Lane 1: Human fetal liver lysate, 20 ug
Lane 2: Human tonsil lysate, 20 ug
Lane 3: Human fetal spleen lysate, 20 ug
Lane 4: U937 (Human histiocytic lymphoma cell line) whole cell lysate, 20 ug
Lane 5: THP-1 (Human monocytic leukemia cell line) whole cell lysate, 20 ug
Lane 6: J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate, 20 ugSecondary (all lanes): ab97051 at 1/100000 dilution.
Predicted MW: 121 kDa.
Observed MW: 150 kDa.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 minute; Lane 2-5: 3 minutes.
U937 , THP-1 and J774A.1 cell lines were reported to be negative for CD163 expression.(PMID:16368951, 10648003 & 10577520).
The molecular weight observed is consistent with what has been described in the literature (PMID:9712057 & 16517975).
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Western blot - Macrophage polarization panel - human WB (ab278181)All lanes: Anti-Mannose Receptor antibody [EPR22489-7] (ab252921) at 1/1000 dilution.
Lane 1: Human liver lysate, 20 ug
Lane 2: Human lung lysate, 20 ug
Lane 3: Human lymph node lysate, 20 ug
Lane 4: Human placenta lysate, 20 ugSecondary (all lanes): ab131366 at 1/1000 dilution.
Predicted MW: 166 kDa.
Observed MW: 175 kDa.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 3 mins. Lanes 2 & 3: 26 secs. Lane 4: 10 secs.
The molecular weight observed is consistent with what has been described in the literature (PMID: 26202986; 23752129).
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Western blot - Macrophage polarization panel - human WB (ab278181)All lanes: Anti-Mannose Receptor antibody [EPR22489-7] (ab252921) at 1/1000 dilution.
Lane 1: Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate, 20 ug
Lane 2: THP-1 treated with 20 ng/ml PMA for 24 hours, then treated with 20ng/ml IL-4 for 48 hours, whole cell lysate, 20 ug
Secondary (all lanes): ab97051 at 1/100000 dilution.
Predicted MW: 166 kDa.
Observed MW: 175 kDa.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight of 175 kDa in lane 2 observed is consistent with what has been described in the literature (PMID: 26202986; 23752129). This antibody also reacts with an unidentifiable protein around 40 kDa.
Exposure: 3 minutes
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Western blot - Macrophage polarization panel - human WB (ab278181)All lanes: Anti-CD68 antibody [EPR20545] (ab213363) at 1/1000 dilution.
Lane 1: Human fetal liver lysate, 20 ug
Lane 2: Human tonsil lysate, 20 ug
Lane 3: Human fetal spleen lysate, 20 ug
Lane 4: THP-1 (human monocytic leukemia cell line) whole cell lysate, 10 ug
Lane 5: U937 (human histiocytic lymphoma cell line) whole cell lysate, 10 ugSecondary (all lanes): ab97051 at 1/100000 dilution.
Predicted MW: 37 kDa.
Observed MW: 110 kDa.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2/4/5: 30 seconds; Lane 3: 3 minutes.
The observed molecular weight is consistent with the literature (PMID:18405323; PMID:11739566; PMID: 16710801).
