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Anti-GLYR1 antibody [EPR10076(B)] (ab154838)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10076(B)] to GLYR1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GLYR1 antibody [EPR10076(B)]
    See all GLYR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10076(B)] to GLYR1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human GLYR1 (Uniprot: Q49A26)

  • Positive control

    • WB: HEK293T, Jurkat, HeLa, K562 and HUVEC cell lysates. IHC-P: Human colon and skin tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)
    Western blot - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)
    All lanes : Anti-GLYR1 antibody [EPR10076(B)] (ab154838) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : GLYR1 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HUVEC cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab154838 observed at 61 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab154838 Anti-GLYR1 antibody [EPR10076(B)] was shown to specifically react with GLYR1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266610 (knockout cell lysate ab257968) was used. Wild-type and GLYR1 knockout samples were subjected to SDS-PAGE. ab154838 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)
    Western blot - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)
    All lanes : Anti-GLYR1 antibody [EPR10076(B)] (ab154838) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling GLYR1 using ab154838 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10076(B)] (ab154838)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue, labeling GLYR1 using ab154838 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-GLYR1 antibody [EPR10076(B)] (ab154838)
    Anti-GLYR1 antibody [EPR10076(B)] (ab154838)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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