Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GLYR1 antibody [EPR10077(B)] (ab167155)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10077(B)] to GLYR1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-GLYR1 antibody [EPR10077(B)]
See all GLYR1 primary antibodies
Description

Rabbit monoclonal [EPR10077(B)] to GLYR1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human GLYR1. The exact sequence is proprietary.
Positive control
- Jurkat, HeLa and K562 cell lysates. Human colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10077(B)
Isotype

IgG
Research areas

Western blot - Anti-GLYR1 antibody [EPR10077(B)] (ab167155)

All lanes : Anti-GLYR1 antibody [EPR10077(B)] (ab167155) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : GLYR1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HUVEC cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa

Lanes 1-4: Merged signal (red and green). Green - ab167155 observed at 61 kDa. Red - loading control ab8245 observed at 36 kDa.

ab167155 Anti-GLYR1 antibody [EPR10077(B)] was shown to specifically react with GLYR1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266610 (knockout cell lysate ab257968) was used. Wild-type and GLYR1 knockout samples were subjected to SDS-PAGE. ab167155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10077(B)] (ab167155)

ab167155 showing +ve staining in Mouse kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10077(B)] (ab167155)

ab167155 showing +ve staining in Human normal stomach.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10077(B)] (ab167155)

ab167155 showing +ve staining in Human thyroid gland papillary carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10077(B)] (ab167155)

ab167155 showing +ve staining in Human ovarian carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10077(B)] (ab167155)

ab167155 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-GLYR1 antibody [EPR10077(B)] (ab167155)

All lanes : Anti-GLYR1 antibody [EPR10077(B)] (ab167155) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 61 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLYR1 antibody [EPR10077(B)] (ab167155)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling GLYR1 with ab167155 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-GLYR1 antibody [EPR10077(B)] (ab167155)

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