Anti-HP1 alpha antibody (ab231559)
Key features and details
- Rabbit polyclonal to HP1 alpha
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HP1 alpha antibody
See all HP1 alpha primary antibodies -
Description
Rabbit polyclonal to HP1 alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant full length protein (GST-tag) corresponding to Human HP1 alpha aa 1 to the C-terminus.
Database link: P45973 -
Positive control
- WB: HeLa nuclear extract. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HP1 alpha antibody (ab231559)Anti-HP1 alpha antibody (ab231559) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear extract at 20 µg
Predicted band size: 22 kDaDilution buffer: TBS-Tween containing 5% skimmed milk.
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Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody (ab231559)HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for HP1 alpha using ab231559 at a dilution of 1/500 (Left panel) in ICC/IF. Cells were fxed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. An anti-rabbit antibody conjugated to Alexa-Fluor®488 was used as the secondary antibody. Central panel is DAPI. Right panel merged images.
