Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)
![Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)](https://www.abcam.com/ps/products/256/ab256541/Images/ab256541-353166-anticollagenIantibodyepr2220975westernblothuman.jpg "Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22209-75] to Collagen I - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free
See all Collagen I primary antibodies -
Description
Rabbit monoclonal [EPR22209-75] to Collagen I - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3, HFF-1 cell culture supernatants; mouse kidney and lung organ culture suparnatanta; rat lung organ culture supernatant.
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General notes
Ab256541 is the carrier-free version of ab255809. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256541 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22209-75 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)All lanes : Anti-Collagen I antibody [EPR22209-75] (ab255809) at 1/1000 dilution
Lane 1 : HFF-1 (human skin fibroblast) whole cell lysate at 20 µg
Lane 2 : HFF-1 culture supernatant at 10 µl
Lane 3 : MRC-5 (human lung fibroblast) whole cell lysate at 20 µg
Lane 4 : MRC-5 culture supernatant at 10 µl
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 139 kDa
Observed band size: 220,35 kDa why is the actual band size different from the predicted?Procollagen I (220 kDa) and cleaved C-propeptide (35 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311).
Exposure time: 3 minutes.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255809).
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![Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)](https://www.abcam.com/ps/products/256/ab256541/Images/ab256541-3-benefits-of-recombinant-antibodies.png)
Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)
