Antibodies, Proteins, Kits and Reagents for Life Science

Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17557] to JNK1 - BSA and Azide free
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Chicken, Hamster, Dog, Human, Monkey, Xenopus tropicalis

Product name

Anti-JNK1 antibody [EPR17557] - BSA and Azide free
See all JNK1 primary antibodies
Description

Rabbit monoclonal [EPR17557] to JNK1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WBmore details
Species reactivity

Reacts with: Mouse, Rat, Chicken, Hamster, Dog, Human, Monkey, Xenopus tropicalis
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251271 is the carrier-free version of ab199380. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251271 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR17557
Isotype

IgG
Research areas

Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

This data was developed using ab199380, the same antibody clone in a different buffer formulation.

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab199380 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab199380 was shown to recognize JNK1 when JNK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and JNK1 knockout samples were subjected to SDS-PAGE. ab199380 and ab8226 (loading control to beta Actin) were diluted to 1/2500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution

Lane 1 : Full length Human JNK1 recombinant protein
Lane 2 : Full length Human JNK2 recombinant protein
Lane 3 : Full length Human JNK3 recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 48 kDa
why is the actual band size different from the predicted?

This data was developed using ab199380, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Recombinant full length JNK1 protein contains aa1-427 with His-Tag^®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.
Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/10000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : U937 (Human histiocytic lymphoma cells) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

This data was developed using ab199380, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution + Human fetal brain lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

This data was developed using ab199380, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

This data was developed using ab199380, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat spleen lysate
Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

This data was developed using ab199380, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

Lane 1 : MDCK (Canine kidney cell line) whole cell lysates
Lane 2 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates
Lane 3 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

This data was developed using ab199380, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

Lane 1 : Xenopus (X. tropicalis) muscle lysates
Lane 2 : BHK (Hamster kidney fibroblast cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

This data was developed using ab199380, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

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