Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17557] to JNK1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Chicken, Hamster, Dog, Human, Monkey, Xenopus tropicalis

Overview

  • Product name

    Anti-JNK1 antibody [EPR17557] - BSA and Azide free
    See all JNK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17557] to JNK1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Hamster, Dog, Human, Monkey, Xenopus tropicalis

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251271 is the carrier-free version of ab199380. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251271 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

    This data was developed using ab199380, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: MCF7 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab199380 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab199380 was shown to recognize JNK1 when JNK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and JNK1 knockout samples were subjected to SDS-PAGE. ab199380 and ab8226 (loading control to beta Actin) were diluted to 1/2500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution

    Lane 1 : Full length Human JNK1 recombinant protein
    Lane 2 : Full length Human JNK2 recombinant protein
    Lane 3 : Full length Human JNK3 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab199380, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Recombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.

  • Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : U937 (Human histiocytic lymphoma cells) whole cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    This data was developed using ab199380, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    This data was developed using ab199380, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    This data was developed using ab199380, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    This data was developed using ab199380, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

    Lane 1 : MDCK (Canine kidney cell line) whole cell lysates
    Lane 2 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates
    Lane 3 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    This data was developed using ab199380, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    Western blot - Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

    Lane 1 : Xenopus (X. tropicalis) muscle lysates
    Lane 2 : BHK (Hamster kidney fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    This data was developed using ab199380, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
    Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)

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